General Cell Based Assays
Molecular Devices Fluorescent Assay Kit, cAMP, 96-Well, Bulk
The CatchPoint cAMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of intracellular or biochemical cAMP levels in cell-based and enzymatic assays. It provides high-affinity reagents and pre-coated microplates optimized for sensitive fluorescence detection.
- Format: 96-well bulk kit with reagents for ten 96-well plates.
- Target analyte: 3',5'-cyclic adenosine monophosphate (cAMP).
- Detection: fluorescent competitive immunoassay, 120 μL total assay volume per well.
- Contents: pre-coated black 96-well plates, rabbit anti-cAMP antibody, HRP-cAMP conjugate, cAMP calibrator, substrate, assay buffer, wash concentrate, cell lysis buffer.
- Incubation: 2 hours for antibody/conjugate incubation; substrate signal readable from 10 minutes up to 24 hours.
- Storage: store components at 4°C; reconstituted reagents stable for about 3 weeks at 4°C.
Eurofins Discoverx cAMP Hunter™ Human CMKLR1 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human CMKLR1 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CMKLR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
BioVendor, LLC Sircol™ Collagen Isolation & Concentration Reagent (3 x 20 ml)
Sircol™ Collagen Isolation & Concentration Reagent (3 x 20 ml). Contains polyethylene glycol in a TRIS-HCl buffer, pH 7.6. Sircol Collagen Assay component. Biocolor Item # SICR
Cell Biolabs Inc OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size, 20 assays
The OxiSelect™ In Vitro ROS/RNS Assay Kit is an assay for measuring the total free radical presence of a sample. The assay employs a proprietary quenched fluorogenic probe, DCFH-DiOxyQ), which is based on similar chemistry to the popular DCFH-DA. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2’, 7’-dichlorodihydrofluorescein (DCF) . The DCFH-DiOxyQ probe can react with hydrogen peroxide, peroxyl radical, nitric oxide, and peroxynitrite anion. These free radical molecules are representative of both ROS and RNS, thus allowing for measurement of the total free radical population within a sample. The kit has a detection sensitivity limit of 40 nM for H2O2. Each trial size kit provides sufficient reagents to perform up to 20 assays, including standard curve and unknown samples.
Revvity Health Sciences Inc HTRF Human Phospho-IRF3 (Ser386) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-IRF3 (Ser386) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF CD32a (131R) Cellular Binding Kit, 500 Assay Points
HTRF CD32a (131R) Cellular Binding Kit, 500 Assay Points
Cayman Chemical HAT Inhibitor Screening Assay
Cayman’s HAT Inhibitor Screening Assay provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three EAsy steps, all performed in the same microplate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3 peptide. During this time, HAT catalyzes the enzymatic transfer of acetyl groups from acetyl-CoA to the H3 peptide producing an acetylated peptide and CoASH. Following addition of isopropanol to stop the enzymatic reaction, CPM is added to the wells of the plate. CPM reacts with the free thiol groups present on CoASH forming a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 and 450-470 nm, respectively.
LifeSensors E3LITE Ubiquitin Ligase PLATE only 384-well
The E3LITE Customizable Ubiquitin Ligase Plate consists of a plate that is pre-coated with a proprietary polyubiquitin capture reagent. The resulting product of the E3 ligase-mediated in vitro ubiquitin reaction is measured and represents a direct measure of the E3 ligase activity. The E3LITE Customizable Ubiquitin Plate represents a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as many polyubiquitin linkage types. This assay has been validated for high throughput screening (HTS) and has been employed in the discovery of novel compounds that selectively modulate E3 ligase function. This product is protected by one or more US or Foreign patents.
Revvity Health Sciences Inc HTRF mAb Anti-6His d2-Conjugate, 5,000 Assay Points
HTRF mAb Anti-6His d2-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human AQ STING Binding Kit, 10,000 Assay Points
HTRF Human AQ STING Binding Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human AQ STING Binding Kit, 100,000 Assay Points
HTRF Human AQ STING Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total FOXO1 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total FOXO1 Detection Kit, 50,000 Assay Points