General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress mGIPR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0154E2CP2L)
The cAMP Hunter mGIPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GIP) based activation of mGIPR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type mGIPR, a naturally Gs coupled GPCR. Upon activation of mGIPR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Electron Microscopy Sciences Flim Sleeve 135 mm/36 exp with Clear open flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Clear open flap
Bioassay Systems QuantiChrom™ Peroxidase Assay Kit. For quantitative determination of peroxidase enzyme activity.
For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H+ → ROH + R'OH.
Eurofins Discoverx PathHunter Human LXR alpha Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human LXR alpha Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated LXRα nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human RXR gamma Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human RXR gamma Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated RXRγ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx AssayComplete Cell Plating 36 Reagent, 100. (93-0563R36A)
AssayComplete Cell Plating 36 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress GLP2R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0572E2CP2L)
The PathHunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 CHO-K1 β-Arrestin-1 GPCR Assay, 10pl Kit. (93-1050E2CP2L)
The PathHunter AGTRL1 (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-1. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology Cathepsin B Rabbit pAb
This gene encodes a member of the C1 family of peptidases. Alternative splicing results in multiple transcript variants, with at least one encoding a preproprotein that is processed into cathepsin B light and heavy chains, which dimerize to form the active enzyme. Cathepsin B is a lysosomal cysteine protease with endopeptidase and exopeptidase activity, involved in protein turnover. It also acts as amyloid precursor protein secretase, with incomplete APP processing linked to Alzheimers disease. Overexpression of this protein is associated with esophageal adenocarcinoma and other cancers. Both cathepsin B and L are involved in cleaving the spike protein of SARS-CoV-2 during cell entry. Multiple pseudogenes of this gene have been identified.
Abcam Lovastatin (Mevinolin), HMG-CoA reductase inhibitor, 50MG
MW 404.5 Da, Purity >98%. Potent, competitive HMG-CoA reductase inhibitor (Ki = 0.6 nM). Blood-brain barrier permeable. Inhibits in vivo and in vitro cholesterol biosynthesis. Also reduces α-synuclein levels in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Mouse IL-4 ASY
The Meso Scale Discovery U-PLEX Mouse IL-4 Assay Kit is a sensitive immunoassay for detecting and quantifying mouse interleukin 4 (IL-4) in samples like serum plasma and cell culture supernatant It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The assay detects IL-4 at 0 03 pg/mL with a 7-log range The kit includes a 96-well plate with capture antibodies detection antibodies and calibrators The assay completes in 3 5 hours after a 2-hour incubation providing a robust tool for research
Abcam MK886, Lipo x ygenase inhibitor, 50MG
MW 494.1 Da, Purity >98%. Potent 5-lipoxygenase activating protein inhibitor (FLAP) (IC₅₀ = 44 nM). Inhibits leukotriene biosynthesis (IC₅₀ = 2.5 nM) in vitro and tumor activity in vivo. Displays PPARα antagonist activity. Orally active in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery R-PLEX Human BMP-6 ASY
The R-PLEX Human BMP-6 Assay Kit from Meso Scale Discovery is an electrochemiluminescent immunoassay for the quantitative measurement of human Bone Morphogenetic Protein 6 (BMP-6) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SECTOR Imager 6000 for high sensitivity detection The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and sample diluent The assay has a range from 1 5 pg/mL to 1000 pg/mL and takes approximately 3 5 hours to complete This product can be used for biomarker research related to bone formation fracture healing and developmental disorders
Meso Scale Discovery R-PLEX Human E-Cadherin ASY
The R-Plex Human E-Cadherin Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures E-Cadherin levels in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay allows for the quantitative measurement of E-Cadherin an important biomarker that plays a role in cell adhesion and is often monitored in cancer research With minimal hands-on time this robust and reproducible assay generates rapid results using automated instrumentation
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 10-Plate Kit. (93-0271E2CP2L)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.